The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. Immunofluorescence analysis of CD79a was performed using 70% confluent log phase Daudi cells. ![]() The images were captured at 60X magnification. ![]() Panel f represents control cells with no primary antibody to assess background. Panel e represents Jurkat cells showing no expression of CD79a. Panel d represents the merged image showing plasma membrane and cytoplasm localization. F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). The cells were labeled with CD79a Monoclonal Antibody (HM57) (Product # MA1-81870) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). ![]()
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